Coding

Part:BBa_K3789009:Design

Designed by: MEI YING YI   Group: iGEM21_UM_Macau   (2021-09-26)


6 x glycine


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We inserted 6 x glycine between Mating factor alpha prepro-leader (MF alpha) and three of our protein-encoding genes (PNP1, SOD1, chit42) to avoid interference between MF alpha and Purine nucleoside phosphorylase, superoxide dismutase 1 enzyme, and endochitinase, so that the correct spatial structure could be formed.



Source

Company synthesis

References